Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDH1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH1 .Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDH1 monoclonal antibody.Application
Anti-IDH1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with IDH1| Product Name | IDH1 antibody [2H9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2H9] to IDH1Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq] |
| Immunogen | Protein expressed in 293T cell transfected with human IDH1 expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2H9 |
| Host Species | Mouse |
| Tested Applications | ICC/IF,IHC-P,WB |
| Recommended Starting Dilutions: For WB : 1:2000. For IHC-P : 1:50. ICC/IF: 1:100. Optimal titers for other applications should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IDH, IDPC, PICD, IDH1, IDP, IDCD |
|---|---|
| Target | IDH1 |
| Tissue Specificity | is specific for human IDH1. |
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDH1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH1 .
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDH1 monoclonal antibody.
Application
Anti-IDH1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with IDH1| Application Notes | Recommended Starting Dilutions: For WB : 1:2000. For IHC-P : 1:50. ICC/IF: 1:100. Optimal titers for other applications should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
