Application
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody [GT1521] diluted at 1:4000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
IDH1 antibody [GT1521] detects IDH1 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: IDH1 protein stained by IDH1 antibody [GT1521] diluted at 1:200. Blue: Hoechst 33342 staining.Application
IDH1 antibody [GT1521] detects IDH1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 10% SDS-PAGE IDH1 antibody [GT1521] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
IDH1 antibody [GT1521] detects IDH1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat-2 whole cell lysate/extract 10% SDS-PAGE IDH1 antibody [GT1521] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
IDH1 antibody [GT1521] detects IDH1 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg Raw264.7 whole cell lysate/extract F. 30 µg C2C12 whole cell lysate/extract 10% SDS-PAGE IDH1 antibody [GT1521] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
IDH-1 antibody immunoprecipitates IDH-1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of IDH1 protein expressing HepG2 cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of IDH-1 protein by 2.5 μg of IDH-1 antibody 10% SDS-PAGE The immunoprecipitated IDH-1 protein was detected by IDH-1 antibody diluted at 1 : 1000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.| Product Name | IDH1 antibody [GT1521] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT1521] to IDH1 (isocitrate dehydrogenase 1 (NADP+), soluble)Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IDH1. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT1521 |
| Host Species | Mouse |
| Tested Applications | ICC/IF,IP,WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human,Mouse,Rat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Sheep, Rhesus Monkey, BovineZebrafish (85%), Xenopus laevis (88%), Sheep (95%), Rhesus Monkey (100%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PICD, IDH, IDP, IDCD, isocitrate dehydrogenase 1 (NADP+), soluble, IDPC |
|---|---|
| Target | IDH1 |
| Cellular Localization | Cytoplasm , Peroxisome |
Application
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody [GT1521] diluted at 1:4000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
IDH1 antibody [GT1521] detects IDH1 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: IDH1 protein stained by IDH1 antibody [GT1521] diluted at 1:200. Blue: Hoechst 33342 staining.
Application
IDH1 antibody [GT1521] detects IDH1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 10% SDS-PAGE IDH1 antibody [GT1521] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
IDH1 antibody [GT1521] detects IDH1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat-2 whole cell lysate/extract 10% SDS-PAGE IDH1 antibody [GT1521] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
IDH1 antibody [GT1521] detects IDH1 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg Raw264.7 whole cell lysate/extract F. 30 µg C2C12 whole cell lysate/extract 10% SDS-PAGE IDH1 antibody [GT1521] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
IDH-1 antibody immunoprecipitates IDH-1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of IDH1 protein expressing HepG2 cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of IDH-1 protein by 2.5 μg of IDH-1 antibody 10% SDS-PAGE The immunoprecipitated IDH-1 protein was detected by IDH-1 antibody diluted at 1 : 1000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
