| Product Name | IFN alpha antibody [144BS] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [144BS] to IFN alphaA member of the type 1 IFN family of related proteins produced by leucocytes and fibroblasts. IFN alpha 1 has antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 144BS |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIP |
| ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. FACS: Use at an assay dependent dilution. GTX13781 has been proven useful for the visualization and enumeration of natural IFN alpha producing cells that resemble type 2 dendritic cells (DC2) by a method described by Svenssonn et al. Upon stimulation of human mononuclear cells with influenza virus it was possible to readily detect these DC2 cells at 0.6% of the total MNC population. However, stimulation of MNC with LPS or SAC failed to give any positive staining IFN alpha cells. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | IFL IFN alpha IFN IFNA1 IFNA13 IFNAP22 interferon alpha 1 Interferon alpha 1/13 precursor Interferon Alpha Interferon alpha D LeIF D |
|---|---|
| Target | IFN alpha |
| Tissue Specificity | binds to but does not neutralize the activity of human IFN alpha subtypes -B, -C, -D and -F or several IFNa B/D, D/B hybrids. |
| Application Notes | ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. FACS: Use at an assay dependent dilution. GTX13781 has been proven useful for the visualization and enumeration of natural IFN alpha producing cells that resemble type 2 dendritic cells (DC2) by a method described by Svenssonn et al. Upon stimulation of human mononuclear cells with influenza virus it was possible to readily detect these DC2 cells at 0.6% of the total MNC population. However, stimulation of MNC with LPS or SAC failed to give any positive staining IFN alpha cells. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline containing 0.02% sodium azide |
