Application
Purified human CD4+ T cells were left non-stimulated (Lane 1), stimulated with anti-CD3 (Lane 2), or stimulated with anti-CD3 and anti-CD28 (Lane 3) for three days. IFN gamma production (pg/ml) was determined by ELISA by coating IFN gamma antibody [2G1] (1:1000), blocking with 1% BSA in PBS, and detection with IFN gamma antibody [B133.5] (1:1000), followed by peroxidase-conjugated avidin and TMB substrate.| Product Name | IFN gamma antibody [2G1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2G1] to IFN gammaThis gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009] |
| Immunogen | Recombinant human IFN gamma |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2G1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA and WB assay dependent. Recommended pairs for sandwich immunoassay: Capture: GTX15624, Detection: GTX15751 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | IFI , IFG , IFNG , interferon, gamma |
|---|---|
| Target | IFN gamma |
Application
Purified human CD4+ T cells were left non-stimulated (Lane 1), stimulated with anti-CD3 (Lane 2), or stimulated with anti-CD3 and anti-CD28 (Lane 3) for three days. IFN gamma production (pg/ml) was determined by ELISA by coating IFN gamma antibody [2G1] (1:1000), blocking with 1% BSA in PBS, and detection with IFN gamma antibody [B133.5] (1:1000), followed by peroxidase-conjugated avidin and TMB substrate.| Application Notes | ELISA and WB assay dependent. Recommended pairs for sandwich immunoassay: Capture: GTX15624, Detection: GTX15751 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20°C |
| Storage Buffer | PBS |
