Application
Western blot analysis of extract from 293 cells using IGF-1R (Phospho-Tyr1280) Antibody .| Product Name | IGF1R (phospho Tyr1280) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IGF-1R (Phospho Tyr1280)This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. Ref.19 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, Ref.21 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 1280 (S-F-Y(p)-Y-S) derived from Human IGF-1R |
| Modification | p-Tyr1280 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD221, IGFIR, IGFR, JTK13, MGC142170, MGC142172, MGC18216, IGF1R, soluble IGF1R variant 2, soluble IGF1R variant 1, insulin-like growth factor 1 receptor, IGF-I receptor, insulin-like growth factor I receptor |
|---|---|
| Target | IGF-1R (Phospho Tyr1280) |
| Molecular Weight(MW) | 200, 95 (note) |
Application
Western blot analysis of extract from 293 cells using IGF-1R (Phospho-Tyr1280) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
