Application
Immunofluorescence staining of methanol-fixed MCF cells using IGF-1R Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IGF-1R Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 293 cells using IGF-1R Antibody and the same antibody preincubated with blocking peptide.Application
Western blot analysis of extracts from 293 cells using IGF-1R(Ab-1161) Antibody and the same antibody preincubated with blocking peptide.| Product Name | IGF1R antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IGF-1RThis receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. |
| Immunogen | Peptide sequence around aa.1159~1163 (D-I-Y-E-T) derived from Human IGF-1R . |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IF,IHC-P,WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD221, IGFIR, IGFR, JTK13, MGC142170, MGC142172, MGC18216, IGF1R, soluble IGF1R variant 2, soluble IGF1R variant 1, insulin-like growth factor 1 receptor, IGF-I receptor, insulin-like growth factor I receptor |
|---|---|
| Target | IGF-1R |
| Molecular Weight(MW) | 200, 95 (note) |
Application
Immunofluorescence staining of methanol-fixed MCF cells using IGF-1R Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IGF-1R Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 293 cells using IGF-1R Antibody and the same antibody preincubated with blocking peptide.
Application
Western blot analysis of extracts from 293 cells using IGF-1R(Ab-1161) Antibody and the same antibody preincubated with blocking peptide.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
