Application
Fig. 1. Western Blotting analysis (non-reducing conditions) of CD222 in whole cell lysate of JURKAT human peripheral blood T cell leukemia cell line. Lane 1: immunostaining with anti-CD222 [MEM-238] Lane 2: immunostaining with Isotype mouse IgG1 control| Product Name | IGF2 receptor antibody [MEM-238] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - 238] to IGF2 ReceptorCD222 is a 250kDa transmembrane protein with a short cytoplasmic tail containing an internalization signal. CD222 was originally identified as a receptor for IGFII and M6P-containing proteins (e.g. lysosomal hydrolases). Lysosomal enzymes are sorted to lysosomes via CD222 either from the Golgi, where the enzymes acquire M6P, or from the extracellular space. The majority of CD222 molecules (approximately 90-95%) are located intracellularly, only 5-10% is present on the cell membrane. The internalization rate seems to be enhanced by ligand induced dimerization of CD222 as well as by phosphorylation of its cytoplasmic serine. CD222 is also a receptor for TGFbeta latency associated peptide (LAP), proliferin and may bind several molecules independently of M6P, including plasminogen, CD87 or retinoic acid. It is involved in activation of latent TGFbeta [PROW]. |
| Immunogen | Vaccinia-M6PR/iGFIIR |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-238 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| For WB (Non-reducing): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMousePrimate |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Insulin like growth factor2 receptor, IGFR2, Cation independent mannose6 phosphate receptor, Cation independent mannose 6 phosphate receptor, M6PR, CD222, IGF2 receptor, Insulin like growth factor 2 receptor, MPRI, CIMPR |
|---|---|
| Target | IGF2 receptor |
| Molecular Weight(MW) | 250 kDa (note) |
| Tissue Specificity | CD222 antigen (human) |
| Cellular Localization | Late endosomal/prelysosomal compartment |
Application
Fig. 1. Western Blotting analysis (non-reducing conditions) of CD222 in whole cell lysate of JURKAT human peripheral blood T cell leukemia cell line. Lane 1: immunostaining with anti-CD222 [MEM-238] Lane 2: immunostaining with Isotype mouse IgG1 control| Application Notes | For WB (Non-reducing): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 15 mM sodium azide |
