| Product Name | IGFBP1 antibody [33627.11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [33627.11] to IGFBP1Insulin like growth factor binding protein 1 (IGFBP1) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). The IGFBP members are cysteine rich proteins with conserved cysteine residues clustered in the amino-terminal and the carboxy terminal regions of the molecule. Contained within IGFBP1 and 2 is an integrin receptor recognition sequence (RGD) that is responsible for promoting cell migration by an IGF independent action. IGFBPs hold a central position in IGF ligand receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. IGFBPs will either inhibit or enhance the biological activities of IGF or act in an IGF independent manner. Post-translational modification of IGFBPs, including phosphorylation and proteolysis, will modify the affinities of the binding proteins for IGF and may indirectly regulate IGF actions. IGFBP1 is expressed in liver, decidua, and kidneys and is the major IGF binding protein in human amnionic fluid. In hepatocytes, IGFBP1 production is regulated at the transcriptional level due to the effects of insulin and corticosteriods. IGFBP1 is the major determinant of the level of free IGF in serum. The expression of IGFBP1 is inhibited by insulin, IGFI, and IGFII and is stimulated by glucocorticoids, thyroid hormone, and epidermal growth factor (EGF), indicating an endocrine function. IGFBP1 shows inhibitory actions on cell proliferation and differentiation, presumably by interfering with the interactions between IGF and the IGF receptor (IGFR). |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 33627.11 |
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| ELISA: Use at a concentration of 2 μg/ml. In the ELISA capture assay, plates are coated with 100 μl/well of the capture antibody (2 μg/ml) in combination with 100 μl/well of a detection antibody (affinity-purified biotinylated polyclonal anti-human IGFBP1 antibody at 100 ng/ml). An ELISA range of 62.4 to 4000 pg/ml may be obtained. Neut: Use at a concentration of 10 - 40 μg/ml. The Neutralization Dose50 (ND50) for this antibody is 10-40 μg/ml in the presence of approximately 5 μg/ml of recombinant human IGFBP1 and approximately 6 ng/mL of recombinant human IGF1, using human MCF7 cells. WB: Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 28 kDa. The detection limit for recombinant human IGFBP1 is approximately 0.5 ng/lane under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | AFBP, IBP1, IGF-BP25, PP12, hIGFBP-1, IGF BP25, hIGFBP 1, IGFBP25, hIGFBP1 |
|---|---|
| Target | IGFBP1 |
| Tissue Specificity | No cross-reactivity is seen with recombinant human IGFBP2, IGFBP3 and IGFBP4. |
| Cellular Localization | Secreted |
| Application Notes | ELISA: Use at a concentration of 2 μg/ml. In the ELISA capture assay, plates are coated with 100 μl/well of the capture antibody (2 μg/ml) in combination with 100 μl/well of a detection antibody (affinity-purified biotinylated polyclonal anti-human IGFBP1 antibody at 100 ng/ml). An ELISA range of 62.4 to 4000 pg/ml may be obtained. Neut: Use at a concentration of 10 - 40 μg/ml. The Neutralization Dose50 (ND50) for this antibody is 10-40 μg/ml in the presence of approximately 5 μg/ml of recombinant human IGFBP1 and approximately 6 ng/mL of recombinant human IGF1, using human MCF7 cells. WB: Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 28 kDa. The detection limit for recombinant human IGFBP1 is approximately 0.5 ng/lane under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None Constituents: PBS |
