Application
Intracellular staining of 10^6 ThP-1 cells using 0.25 ug. Shaded histogram represents cells alone, green represents the isotype control, and red represents the IkBa antibody. IMGENEX's intracellular flow kit was used for this test, and an anti-mouse IgG FITC conjugated secondary.Application
Formalin-fixed, paraffin-embedded human breast probed with IkBa antibody at 5 ug/ml. IMGENEXs human tissue TMA was used for this test.Application
ApplicationApplication
Western blot analysis of IkBa at 2 ug/ml in (A) Daudi and (B) NIH 3T3 whole cell lysate.| Product Name | IKB alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [6A920] to IKB alpha |
| Immunogen | 6xHis-tagged fusion protein, corresponding to amino acids 105-291 of Human IKB alpha. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6A920 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-PIPWB |
| For IP: Use at a concentration of 1 μg/ml. For WB: Use at a concentration of 1-2 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Target | IKB alpha |
|---|---|
| Cellular Localization | Cytoplasmic |
Application
Intracellular staining of 10^6 ThP-1 cells using 0.25 ug. Shaded histogram represents cells alone, green represents the isotype control, and red represents the IkBa antibody. IMGENEX's intracellular flow kit was used for this test, and an anti-mouse IgG FITC conjugated secondary.
Application
Formalin-fixed, paraffin-embedded human breast probed with IkBa antibody at 5 ug/ml. IMGENEXs human tissue TMA was used for this test.
Application
Application
Application
Western blot analysis of IkBa at 2 ug/ml in (A) Daudi and (B) NIH 3T3 whole cell lysate.| Positive Control | Daudi, Jurkat, or NIH 3T3 |
|---|---|
| Application Notes | For IP: Use at a concentration of 1 μg/ml. For WB: Use at a concentration of 1-2 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline containing 0.05% BSA and 0.05% sodium azide |
