Application
Flow cytometric analysis of IKK epsilon in 10^6 NIH 3T3 cells using 0.1 ug. Shaded histogram represents cells without antibody; green represents isotype control; red represents IKK epsilon antibody. A goat anti-mouse IgG: FITC secondary antibody was used for this test.Application
Western blot analysis of A) human Daudi, B) HeLa, and C) mouse NIH3T3 lysate probed with IKKbeta antibody at 2 ug/ml.Application
Intracellular staining of HEK293 cells with 0.5 ug of IKKbeta antibody (red) and 0.5 ug of isotype control antibody (green). IMGENEX's intracellular flow kit was used for this test, and an anti-mouse IgG1 PE conjugated secondary.Application
Formalin-fixed, paraffin-embedded human gall bladder probed with IKKbeta antibody at 5 ug/ml. IMGENEXs human tissue TMA was used for this test.Application
Western blot analysis of IKKi at 2 ug/ml on 15 ugs of Jurkat cell lysateApplication
IKK iota/IKK epsilon (80 kDa) detection by Western blot. The analysis of 10 g of total cell extract from Daudi cells with the anti-IKK iota/IKK epsilon at 0.5 g/ml (lane 1) and 3 g/ml (lane 2) dilution| Product Name | IKBKE antibody [72B587] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [72B587] to IKBKEThis member of the Ser/Thr protein kinase family (IkappaB kinase subfamily) phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. It is highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta, and may play a special role in the immune response; it is weakly expressed in lung, kidney, prostate, ovary and colon. |
| Immunogen | A synthetic peptide corresponding to amino acid residues 175-188, 525-540, or 567-580 of human IKKe/IKKi. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 72B587 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-PWB |
| WB: Use at a concentration of 1 - 3 μg/ml. Detects a band of approximately 80 kDa. For optimal results, primary antibody incubations should be performed at room temperature. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 72B587, IKKi/IKKeIkBKE, IKK epsilon, IKKi/ IKKe IKKi / IKKe, IKKi, IKKI, Inducible I kappa B kinase, IKK i / IKK e, inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon, Inhi, IKK-E, IKK-i, IKKE, KIAA0151, MGC125294, MGC125295, MGC |
|---|---|
| Target | IKKe/IKKi/TBK1 |
| Cellular Localization | Cytoplasmic |
Application
Flow cytometric analysis of IKK epsilon in 10^6 NIH 3T3 cells using 0.1 ug. Shaded histogram represents cells without antibody; green represents isotype control; red represents IKK epsilon antibody. A goat anti-mouse IgG: FITC secondary antibody was used for this test.
Application
Western blot analysis of A) human Daudi, B) HeLa, and C) mouse NIH3T3 lysate probed with IKKbeta antibody at 2 ug/ml.
Application
Intracellular staining of HEK293 cells with 0.5 ug of IKKbeta antibody (red) and 0.5 ug of isotype control antibody (green). IMGENEX's intracellular flow kit was used for this test, and an anti-mouse IgG1 PE conjugated secondary.
Application
Formalin-fixed, paraffin-embedded human gall bladder probed with IKKbeta antibody at 5 ug/ml. IMGENEXs human tissue TMA was used for this test.
Application
Western blot analysis of IKKi at 2 ug/ml on 15 ugs of Jurkat cell lysate
Application
IKK iota/IKK epsilon (80 kDa) detection by Western blot. The analysis of 10 g of total cell extract from Daudi cells with the anti-IKK iota/IKK epsilon at 0.5 g/ml (lane 1) and 3 g/ml (lane 2) dilution| Application Notes | WB: Use at a concentration of 1 - 3 μg/ml. Detects a band of approximately 80 kDa. For optimal results, primary antibody incubations should be performed at room temperature. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
