Application
IKK beta antibody detects IKK beta protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of DDDDK-human IKBKB-transfected 293T cells 7.5 % SDS-PAGE IKK beta antibody dilution: 1:5000Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IKK beta antibody at 1:500 dilution.Application
IKK beta antibody detects IKBKB protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 7.5 % SDS-PAGE IKK beta antibody dilution: 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using IKK beta antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:1000| Product Name | IKK beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IKK beta (inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta)NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human IKK beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Pig, Chicken, Rhesus Monkey, BovineMouse (95%), Pig (92%), Chicken (82%), Rhesus Monkey (100%), Bovine (92%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ33771 antibody FLJ36218 antibody FLJ38368 antibody FLJ40509 antibody IKK-beta antibody IKK2 antibody IKKB antibody MGC131801 antibody NFKBIKB antibody IKBKB antibody IKK-B antibody I-kappa-B kinase 2 antibody I-kappa-B-kinase beta antibody |
|---|---|
| Molecular Weight(MW) | 87 kDa (note) (4) |
| Cellular Localization | Cytoplasm |
Application
IKK beta antibody detects IKK beta protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of DDDDK-human IKBKB-transfected 293T cells 7.5 % SDS-PAGE IKK beta antibody dilution: 1:5000
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IKK beta antibody at 1:500 dilution.
Application
IKK beta antibody detects IKBKB protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 7.5 % SDS-PAGE IKK beta antibody dilution: 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using IKK beta antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:1000| Positive Control | A431 , PC-12 , DDDDK-human IKBKB-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
