Application
IKK beta antibody detects IKBKB protein by Western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg K562 whole cell lysate/extract D. 30 µg THP-1 whole cell lysate/extract E. 30 µg NCI-H929 whole cell lysate/extract 10 % SDS-PAGE IKK beta antibody dilution: 1:1000Application
Immunoprecipitation of IKK beta protein from A431 whole cell extracts using 5 μg of IKK beta antibody . Western blot analysis was performed using IKK beta antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
IKK beta antibody detects IKK beta protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. IKK beta antibody diluted at 1:500.Application
IKK beta antibody detects IKK beta protein at cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IKK beta protein stained by IKK beta antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | IKK beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IKK beta (inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta)NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IKK beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ33771 antibody FLJ36218 antibody FLJ38368 antibody FLJ40509 antibody IKK-beta antibody IKK2 antibody IKKB antibody MGC131801 antibody NFKBIKB antibody IKBKB antibody IKK-B antibody I-kappa-B kinase 2 antibody I-kappa-B-kinase beta antibody |
|---|---|
| Molecular Weight(MW) | 87 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
IKK beta antibody detects IKBKB protein by Western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg K562 whole cell lysate/extract D. 30 µg THP-1 whole cell lysate/extract E. 30 µg NCI-H929 whole cell lysate/extract 10 % SDS-PAGE IKK beta antibody dilution: 1:1000
Application
Immunoprecipitation of IKK beta protein from A431 whole cell extracts using 5 μg of IKK beta antibody . Western blot analysis was performed using IKK beta antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
IKK beta antibody detects IKK beta protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. IKK beta antibody diluted at 1:500.
Application
IKK beta antibody detects IKK beta protein at cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IKK beta protein stained by IKK beta antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | Jurkat , Raji , K562 , THP-1 , NCI-H929 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
