Application
Western blot analysis of IKK beta in Daudi cell lysate using IKK beta antibody at 5 ug/ml.Application
2 g of was used to immunoprecipitate IKK beta from 1x10^6 cells. The immuno-precipitated protein was detected by western blotting (ECL).| Product Name | IKK beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [10A9B6] to IKK betaNFkB is associated with IkB proteins in the cell cytoplasm, which inhibit NFkB activity. The IkB kinase (IKK) phosphorylates IkB, and mediates IkB degradation and NFkB activation. IKK is a serine protein kinase. The beta subunit is part of the IKK complex. |
| Immunogen | This antibody was raised against the 6xHis tag full-length proteincorresponding to human IKK beta. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 10A9B6 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| IP: 2μg of antibody is recommended for performing immunoprecipitation. WB: Use at a concentration of 1-2 μg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 90.7 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | FLJ33771 FLJ36218 FLJ38368 FLJ40509 IKK-beta IKK2 IKKB MGC131801 NFKBIKB IKK beta IKKbeta |
|---|---|
| Target | IKK beta |
| Cellular Localization | Cytoplasmic |
Application
Western blot analysis of IKK beta in Daudi cell lysate using IKK beta antibody at 5 ug/ml.
Application
2 g of was used to immunoprecipitate IKK beta from 1x10^6 cells. The immuno-precipitated protein was detected by western blotting (ECL).| Positive Control | Daudi whole-cell extract |
|---|---|
| Application Notes | IP: 2μg of antibody is recommended for performing immunoprecipitation. WB: Use at a concentration of 1-2 μg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 90.7 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
