Application
Flow cytometric analysis using NEMO (IKKgamma) antibody.Human Jurkat cells were probed using 0.1 ug of NEMO antibody (red) and 0.1 ug of isotype control (green), with shaded histogram representing cells alone. IMGENEX’s intracellular flow kit was used for this test, and an anti-mouse IgG-FITC secondary antibody.Application
Western blot analysis of 30 g of total cell lysate from Jurkat cells with at 2 ug/ml dilution.| Product Name | IKK gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [46B844] to IKK gammaNuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. The IkB kinase (IKK alpha and IKK beta)phosphorylates IkB and mediates NF-kB activation. A novel molecule in the IKK complex was recently identified and termed IKK gamma. IKK gamma interacts with IKK beta and is required for the activation of IKK complex and NF-kB by LPS, PMA, TNF, and IL-1 stimulation. IKK gamma has also been shown to bind to RIP and NIK and to mediate TNF-induced NF-kB activation. |
| Immunogen | Full length native protein (purified) (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 46B844 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 48 kDa from whole cell extract of Daudi cells. For optimal results, primary antibody incubations should be performed at room temperature. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 12141 GTX12141 46B844 IKK gamma IKK gamma 46B844 46B844 IKK gamma Ab12141 46B844 IKK gamma IKK gamma 46B844 FIP3 antibody Fip3p antibody I kappa B kinase gamma antibody IkB kinase associated protein 1 antibody IkB kinase gamma subunit antibody Inhibitor of |
|---|---|
| Target | IKK gamma |
| Cellular Localization | Cytoplasmic and Nuclear |
Application
Flow cytometric analysis using NEMO (IKKgamma) antibody.Human Jurkat cells were probed using 0.1 ug of NEMO antibody (red) and 0.1 ug of isotype control (green), with shaded histogram representing cells alone. IMGENEX’s intracellular flow kit was used for this test, and an anti-mouse IgG-FITC secondary antibody.
Application
Western blot analysis of 30 g of total cell lysate from Jurkat cells with at 2 ug/ml dilution.| Positive Control | Daudi whole cell extract |
|---|---|
| Application Notes | WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 48 kDa from whole cell extract of Daudi cells. For optimal results, primary antibody incubations should be performed at room temperature. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
