Application
An ELISA plate is coated with 50 μL of a IL10 peptide at concentration ranged from 0.25 μg/mL to 8 μg/mL. The coated peptide is detected with anti IL10 antibody at 0.5 μg/mL.Application
IL10 antibody detects IL10 protein by western blot analysis. Non-transfected (-) and IL10-transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL10 antibody diluted at 1:5000.| Product Name | IL10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to IL10The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human IL10. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, ChimpanzeeRhesus Monkey (100%), Chimpanzee (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TGIF IL10A IL-10 CSIF interleukin 10 GVHDS |
|---|---|
| Target | IL10 |
| Molecular Weight(MW) | 21 kDa (note) |
| Cellular Localization | Secreted |
Application
An ELISA plate is coated with 50 μL of a IL10 peptide at concentration ranged from 0.25 μg/mL to 8 μg/mL. The coated peptide is detected with anti IL10 antibody at 0.5 μg/mL.
Application
IL10 antibody detects IL10 protein by western blot analysis. Non-transfected (-) and IL10-transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL10 antibody diluted at 1:5000.| Positive Control | 293T , human IL10-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
