WB
IL-10 antibody: Western blot analysis of human recombinant IL-10.WB
IL-10 antibody: Immunoblotting analysis of IL-10 expression in SK-BR-3 whole cell lysates.IHC
IL-10 antibody: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human gallbladder tissue showed cytoplasmic and membrane staining of glandular cells.| Product Name | Mouse Monoclonal antibody to IL-10 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | amino acids 19-178 of IL-10 of human origin. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2b,k |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 ELISA:1:1000-1:10000 |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL-10 |
|---|---|
| Gene Synonyms | CSIF TGIF GVHDS IL-10 IL10A |
| Gene Full Name | interleukin 10 |
| Gene Summary | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, May 2020] |
| Molecular Weight(MW) | Molecular Weight of IL-10 monomer: 20 kDa. Molecular Weight of IL-10 dimer: 37 kDa. |
| Cellular Localization | Secreted |
WB
IL-10 antibody: Western blot analysis of human recombinant IL-10.
WB
IL-10 antibody: Immunoblotting analysis of IL-10 expression in SK-BR-3 whole cell lysates.
IHC
IL-10 antibody: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human gallbladder tissue showed cytoplasmic and membrane staining of glandular cells.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
