Application
IL-17R Antibody detects IL-17R protein by Western blot analysis. Whole cell extracts of K562 cell line was separated by SDS-PAGE, and the membrane was blotted with anti-IL-17R antibody.| Product Name | IL17 Receptor alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [8D15] to IL-17 RInterleukin 17A (IL17A)is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The protein encoded by this gene (interleukin 17A receptor; IL17RA) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. [provided by RefSeq, Jul 2008] |
| Immunogen | Human recombinant IL-17R EC domain |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 8D15 |
| Host Species | Mouse |
| Tested Applications | NeutWB |
| We recommend the following starting dilutions:
For WB: Use at a dilution of 1:500-1:1000.
For Neutralization:Recombinant Human (rh)IL 17 can induce GRO alpha secretion in the human K562 cancer cells in a dose dependent manner. GRO alpha secretion elicited by rhIL 17 can be neutralized by increasing concentrations of GTX52824. When 50ng/ml of rhIL-17 was used, the ED50 is typically at 500-2000 ng/mL.
Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | interleukin 17 receptor A hIL-17R IL-17RA IL17RA CD217 CDw217 CANDF5 IL17R |
|---|---|
| Target | IL-17 R |
| Tissue Specificity | This antibody was selected for its ability to detect human IL-17R. |
Application
IL-17R Antibody detects IL-17R protein by Western blot analysis. Whole cell extracts of K562 cell line was separated by SDS-PAGE, and the membrane was blotted with anti-IL-17R antibody.| Application Notes | We recommend the following starting dilutions:
For WB: Use at a dilution of 1:500-1:1000.
For Neutralization:Recombinant Human (rh)IL 17 can induce GRO alpha secretion in the human K562 cancer cells in a dose dependent manner. GRO alpha secretion elicited by rhIL 17 can be neutralized by increasing concentrations of GTX52824. When 50ng/ml of rhIL-17 was used, the ED50 is typically at 500-2000 ng/mL.
Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
