| Product Name | IL2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [N7.48A] to IL2Human interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. |
| Immunogen | Synthetic peptide (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | N7.48A |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-P |
| FACS: Use at an assay dependent dilution. Mitogen stimulated purified T cells treated with saponin yield a strong positive signal. ICC: Use 0.5μg for 0.5X10^6 cells permeablized with saponin. IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol (5 min at 120°C). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | IL-2 TCGF lymphokine IL 2 IL2 |
|---|---|
| Target | IL2 |
| Cellular Localization | Secreted |
| Application Notes | FACS: Use at an assay dependent dilution. Mitogen stimulated purified T cells treated with saponin yield a strong positive signal. ICC: Use 0.5μg for 0.5X10^6 cells permeablized with saponin. IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol (5 min at 120°C). Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
