| Product Name | IL2 Receptor alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [B1.49.9] to CD25The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq] |
| Immunogen | Human alloactivated T lymphocytes (FC2) kept in conditioned medium. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | B1.49.9 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-F |
| IHC-Fr: 1:25 to 1:50.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD25 IL2R TCGFR |
|---|---|
| Target | CD25 |
| Tissue Specificity | The molecular weight of the recognized antigen is 55kDa. The IL-2 receptor consists of at least three distinct membrane components: the alpha chain (IL-2R alpha, Tac, CD25), the beta chain (IL-2R beta, p75, CD122), and the gamma chain (IL-2R gamma). CD25 is undetectable on resting T cells but is induced upon T-cell activation. B1.49.9 recognizes activated T and B cells. CD25 is absent on thymocytes, resting T and B cells, and NK cells. B1.49.9 does not inhibit the in vitro IL2 dependent proliferation |
| Light Chain Type | kappa |
| Application Notes | IHC-Fr: 1:25 to 1:50.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at –20°C. Avoid multiple freeze/thaw cycles. |
| Storage Buffer | Lyophilized from PBS containing 1 mg/mL BSA. |
