Application
- immunohistochemistry: Formalin fixed paraffin embedded human tonsil stained with CD25, using ABC and AEC chromagen.| Product Name | IL2 Receptor alpha antibody [IL2R.1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [IL2R.1] to IL2 Receptor alphaThe interleukin 2 receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low affinity receptor, while homodimeric beta (IL2RB) chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. |
| Immunogen | BALB/C mice were injected with recombinant human interleukin-2 receptor. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | IL2R.1 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| Immunohistochemistry: use at a dilution of 1 / 20 - 1 / 50 for 30 - 60 mins (ABC method). Optimal dilutions / concentrations should be determined by the end user. For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval using 10mM citrate buffer pH6.0 before commencing with the IHC staining protocol. | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IL2 R alpha, IL2R alpha, CD25, IDDM10, IL2R, TCGFR |
|---|---|
| Target | IL2-R alpha |
| Tissue Specificity | This antibody is specific to a 55 kDa protein known as interleukin 2-receptor (IL-2R alpha). IL-2R alpha is over expressed on the surface of activated T cell, HTLV-1 or HTLV-2 positive T cell lines and moderately expressed on activated B cells, NK-cells and macrophages. |
| Cellular Localization | Cell Membrane |
| Light Chain Type | kappa |
Application
- immunohistochemistry: Formalin fixed paraffin embedded human tonsil stained with CD25, using ABC and AEC chromagen.| Positive Control | Tonsil |
|---|---|
| Application Notes | Immunohistochemistry: use at a dilution of 1 / 20 - 1 / 50 for 30 - 60 mins (ABC method). Optimal dilutions / concentrations should be determined by the end user. For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval using 10mM citrate buffer pH6.0 before commencing with the IHC staining protocol. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 1% BSA and 0.09% sodium azide |
