Application
Western blot analysis of IL-21R in 30 ugs of Raji cell lysate using at 1 ug/ml.| Product Name | IL21 Receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to IL21 ReceptorThe protein encoded by this gene is a cytokine receptor for interleukin 21 (IL21). It belongs to the type I cytokine receptors, and has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2 (IL2) and interleukin 5 (IL5). This receptor transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3. Knockout studies of a similar gene in mouse suggest a role for this gene in regulating immunoglobulin production. Three alternatively spliced transcript variants encoding the same protein have been described. |
| Immunogen | Synthetic peptides (CSLHRSAHNATHATYT & CDFTSPGDEGPPRSYLR) corresponding to amino acids 65-80 and 505-521 of Human IL21R respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| For ELISA: Use at a concentration of 0.5 - 2 μg/ml. For WB: Use at a concentration of 0.5 - 2 μg/ml. Detects a band of approximately 60 kDa. The 60 kD IL21 Receptor band is detected in 30 ug/lane of whole Raji cell lysate. Bands at 45 kD and 35 kD are also seen. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | IL21 R IL21R MGC10967 NILR |
|---|---|
| Target | IL21-R |
| Cellular Localization | Cell Membrane |
Application
Western blot analysis of IL-21R in 30 ugs of Raji cell lysate using at 1 ug/ml.| Positive Control | Raji or NIH 3T3 |
|---|---|
| Application Notes | For ELISA: Use at a concentration of 0.5 - 2 μg/ml. For WB: Use at a concentration of 0.5 - 2 μg/ml. Detects a band of approximately 60 kDa. The 60 kD IL21 Receptor band is detected in 30 ug/lane of whole Raji cell lysate. Bands at 45 kD and 35 kD are also seen. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline containing 0.2% gelatin and 0.05% sodium azide |
