Application
ELISA analysis of control antigen and antigen using IL3RA antibody [10B8E7].Application
FACS analysis of HeLa cells using IL3RA antibody [10B8E7] (green) or a negative control (red).Application
ICC/IF analysis of HeLa cells using IL3RA antibody [10B8E7] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
WB analysis of lysates from (1) untransfected and (2) IL3RA (a.a. 200-305)-hIgGFc transfected HEK293 cells using IL3RA antibody [10B8E7].Application
WB analysis of human IL3RA recombinant protein using IL3RA antibody [10B8E7].| Product Name | IL3 Receptor alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [10B8E7] to IL3RAThe protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jun 2012] |
| Immunogen | The immunogen corresponding to a region within amino acids 200 and 305 of human IL3RA expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 10B8E7 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HIL-3RA CD123 IL3RAY IL3RX IL3R IL3RY |
|---|---|
| Target | IL3RA |
| Molecular Weight(MW) | 43.3 kDa (note) |
Application
ELISA analysis of control antigen and antigen using IL3RA antibody [10B8E7].
Application
FACS analysis of HeLa cells using IL3RA antibody [10B8E7] (green) or a negative control (red).
Application
ICC/IF analysis of HeLa cells using IL3RA antibody [10B8E7] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
WB analysis of lysates from (1) untransfected and (2) IL3RA (a.a. 200-305)-hIgGFc transfected HEK293 cells using IL3RA antibody [10B8E7].
Application
WB analysis of human IL3RA recombinant protein using IL3RA antibody [10B8E7].| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
