Application
Western blot analysis of IL-32 in human spleen cell lysate with IL-32 antibody at (A) 5 and (B) 10 ug/ml shows two isoforms of IL-32.Application
Immunohistochemistry of IL-32 in human spleen tissue with IL-32 antibody at 10 ug/ml.Application
Immunofluorescence of IL-32 in Human Spleen cells with IL-32 antibody at 20 ug/mL.| Product Name | IL32 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to IL-32Interleukin-32 (IL-32) was initially identified as a transcript (NK4) that is selectively expressed in lymphocytes and NK cells and whose expression is increased following activation by IL-2 . It was later re-isolated from an IL-18-treated lung carcinoma cell line and re-named IL-32 (2). IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. Because treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-alpha, IL-8 and MIP-2, and because it is also induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFNgamma, it has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis (3). |
| Immunogen | Human IL-32 (C-Terminus) Peptide |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Interelukin32 IL32 IL-32 Interelukin 32 IL 32 |
|---|---|
| Target | IL-32 |
| Tissue Specificity | This antibody is specific for IL-32 |
Application
Western blot analysis of IL-32 in human spleen cell lysate with IL-32 antibody at (A) 5 and (B) 10 ug/ml shows two isoforms of IL-32.
Application
Immunohistochemistry of IL-32 in human spleen tissue with IL-32 antibody at 10 ug/ml.
Application
Immunofluorescence of IL-32 in Human Spleen cells with IL-32 antibody at 20 ug/mL.| Positive Control | Blocking Peptide: Cat. No. GTX85070-PEP - IL-32 Peptide Lysates: Cat. No. GTX27921 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Antibody is supplied in PBS containing 0.02% sodium azide |
