Application
Immunohistochemistry of IL-33 in human lymph node tissue with IL-33 antibody at 5 μg/mL.Application
Western blot analysis of IL-33 using 125ng of recombinant IL-33 with IL-33 antibody at 1 μg/mL.| Product Name | IL33 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to IL-33 [12H6D12]Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1alphabeta, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-kappaB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. |
| Immunogen | Mouse monoclonal IL-33 antibody was raised against the recombinant IL-33 protein. |
| Clonality | Monoclonal |
|---|---|
| Clone Number | 12H6D12 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-PWB |
| IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IL-33 (12H6D12) Interleukin33 NFHEV Nuclear factor from high endothelial venules Interleukin-33 IL33 (12H6D12) IL33 Interleukin 33 IL 33 (12H6D12) |
|---|---|
| Target | IL-33 |
| Tissue Specificity | This antibody is specific for IL-33 |
Application
Immunohistochemistry of IL-33 in human lymph node tissue with IL-33 antibody at 5 μg/mL.
Application
Western blot analysis of IL-33 using 125ng of recombinant IL-33 with IL-33 antibody at 1 μg/mL.| Application Notes | IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Antibody is supplied in PBS containing 0.02% sodium azide |
