Application
The Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IL-33 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Product Name | IL33 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4E9 ] to IL33Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1. |
| Immunogen | Recombinant human IL-33 (112-270aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 4E9 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| Western Blot: Use at 1:500 ~ 1,000.Optimal working concentrations should be determined experimentally by the end user.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | INTERLEUKIN33 NFHEV DVS27 related protein IL1F11 Interleukin 33 CHROMOSOME 9 OPEN READING FRAME 26 NF HEV Interleukin 1 family member 11 IL33 Interle RP11 575C20.2 Interleukin33 C9orf26 RP11575C20.2 IL 33 DKFZp586H0523 INTERLEUKIN 33 NFHEV I |
|---|---|
| Target | IL33 |
| Light Chain Type | kappa |
Application
The Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IL-33 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Application Notes | Western Blot: Use at 1:500 ~ 1,000.Optimal working concentrations should be determined experimentally by the end user.: |
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| Form | Liquid |
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| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
