Application
C. Immunofluorescent staining of HeLa cells using anti-ILK RabMAb (cat. #).Application
A. Western blot analysis on (A) HeLa (B) 293T and (C) Jurkat cell lysates using anti-ILK RabMAb (cat. # ) dilution 1:50000.Application
B. Immunofluorescent staining of HeLa cells using anti-ILK RabMAb (cat. #).| Product Name | ILK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EPR1592] to ILKIntegrin-linked kinase (ILK) is a key cytoplasmic plaque protein involved in the mediation of integrin functions. It plays a key role as a regulator during cell survival, cell proliferation, cell migration, and cell to cell adhesion . ILK links integrins to the actin cytoskeleton, allowing transduction of signals through integrins to the extracellular matrix. Also, ILK has been linked to the regulation of smooth contraction and cell motility in non-muscle cells through phosphorylation of myosin . Irregular activity level of ILK has been linked to expression of certain tumor. ILK overexpression caused by inactivating mutation in tumor suppressors (e.g. PTEN and APC) lead to tumor formation in transgenic mouse. Up-regulated ILK expression and activity are seen in Ewings scaromas, colorectal cancers, and late staged prostate cancers . |
| Immunogen | A synthetic peptide corresponding to residues in human ILK was used as an immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR1592 |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFWB |
| For WB: Use at a dilution of 1:5,000 - 50,000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | DKFZp686F1765 P59 ILK ILK-2 ILK 2 ILK2 |
|---|---|
| Target | ILK |
| Molecular Weight(MW) | 51 (note) |
Application
C. Immunofluorescent staining of HeLa cells using anti-ILK RabMAb (cat. #).
Application
A. Western blot analysis on (A) HeLa (B) 293T and (C) Jurkat cell lysates using anti-ILK RabMAb (cat. # ) dilution 1:50000.
Application
B. Immunofluorescent staining of HeLa cells using anti-ILK RabMAb (cat. #).| Application Notes | For WB: Use at a dilution of 1:5,000 - 50,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
