Application
ELISA analysis of control antigen and antigen using INCENP antibody [3D2].Application
FACS analysis of Jurkat cells using INCENP antibody [3D2] (green) or a negative control (red).Application
ICC/IF analysis of HepG2 cells using INCENP antibody [3D2] (green) and DRAQ5 fluorescent DNA dye (blue).Application
IHC-P analysis of cervical cancer tissues using INCENP antibody [3D2].Application
IHC-P analysis of rectum cancer tissues using INCENP antibody [3D2].Application
WB analysis of lysates from (1) untransfected and (2) INCENP (a.a. 369-583)-hIgGFc transfected HEK293 cells using INCENP antibody [3D2].Application
WB analysis of human INCENP recombinant protein using INCENP antibody [3D2].| Product Name | INCENP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [3D2] to INCENPIn mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle pro |
| Immunogen | The immunogen corresponding to a region within amino acids 369 and 583 of human INCENP expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3D2 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Target | INCENP |
|---|---|
| Molecular Weight(MW) | 105.4 kDa (note) |
Application
ELISA analysis of control antigen and antigen using INCENP antibody [3D2].
Application
FACS analysis of Jurkat cells using INCENP antibody [3D2] (green) or a negative control (red).
Application
ICC/IF analysis of HepG2 cells using INCENP antibody [3D2] (green) and DRAQ5 fluorescent DNA dye (blue).
Application
IHC-P analysis of cervical cancer tissues using INCENP antibody [3D2].
Application
IHC-P analysis of rectum cancer tissues using INCENP antibody [3D2].
Application
WB analysis of lysates from (1) untransfected and (2) INCENP (a.a. 369-583)-hIgGFc transfected HEK293 cells using INCENP antibody [3D2].
Application
WB analysis of human INCENP recombinant protein using INCENP antibody [3D2].| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
