Application
Detection of mouse inducible nitric oxide synthase by immunoblotting. Sample: Mouse macrophage RAW 264.7 cell line induced with IFNγ and LPS. Primary antibody: Anti-Nitric Oxide Synthase| Product Name | iNOS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to NOS - II ( iNOS )Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. |
| Immunogen | Mouse macrophage NOS C-terminal peptide (1131-1144) + additional N-terminal Cys conjugated to KLH (CKKGSALEEPKATRL). |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| WB:1:500~1:2000 IHC:1:50~1:200 ICC/IF:1:100~1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NOS-II Nos-2 Nos2a i-NOS iNOS Nos 2 i NOS NOS II Nos2 NOSII |
|---|---|
| Target | iNOS |
| Tissue Specificity | Recognizes inducible nitric oxide sythase (iNOS), and does not cross react with endothelial nitric oxide synthase (eNOS) or brain nitric oxide synthase (bNOS). |
Application
Detection of mouse inducible nitric oxide synthase by immunoblotting. Sample: Mouse macrophage RAW 264.7 cell line induced with IFNγ and LPS. Primary antibody: Anti-Nitric Oxide Synthase| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 ICC/IF:1:100~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH7.2 containing 0.09% sodium azide |
