Rabbit polyclonal to iNOSNOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Notric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. Inducible NOS (iNOS) or macrophage NOS (mNOS) is a calcium/calmodulin independent and is expressed in activated macrophages and stimulated glial cells.
Immunogen
Synthetic peptide derived from C-terminal of mouse iNOS.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHC-P
For IHC-P: Use at a dilution of 1:100 for 10 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the researcher.:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
NOS-II Nos-2 Nos2a i-NOS iNOS Nos 2 i NOS NOS II Nos2 NOSII
Target
iNOS
Cellular Localization
Cytoplasmic
Application
Application
Human lung stained with in IHC paraffin fixed sections
Application
Human Lung stained with anti-Nitric Oxide Synthase-Inducible (iNOS) antibody
Positive Control
Lung
Application Notes
For IHC-P: Use at a dilution of 1:100 for 10 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the researcher.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.
Storage Buffer
1% BSA in PBS (pH 7.6) with less than 0.1% sodium azide