Application
Formalin fixed paraffin embedded human pancreas stained with Insulin antibody (cat. # ).Application
IHC Image using - Detection of Insulin by IHC in Human Pancreas| Product Name | Insulin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to InsulinInsulin is a hormone secreted by the beta cells of the islets of Langerhans in the pancreas. Insulin is a 51 amino acid polypeptide composed of A and B chains connected through the C-peptide. Insulin promotes glycogen storage, formation of triglycerides and synthesis of protein and nucleic acids. Cross reaction has been observed with insulin containing cells in fixed sections of pancreas from human, cow, pig, rabbit and rat. |
| Immunogen | Swine insulin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | E2E3 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-P |
| This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. No pretreatment is required for paraffin sections.: |
|
| Species Reactivity | HumanPig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Insulin |
|---|---|
| Cellular Localization | Cytoplasmic |
Application
Formalin fixed paraffin embedded human pancreas stained with Insulin antibody (cat. # ).
Application
IHC Image using - Detection of Insulin by IHC in Human Pancreas| Positive Control | Pancreas |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. No pretreatment is required for paraffin sections.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
