Application
Western Blot using IR/IGF1R [pYpYpY1158/1162/1163] Polyclonal Antibody, Rabbi| Product Name | Insulin Receptor (phospho Tyr1158/Tyr1162/Tyr1163) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Insulin Receptor (phospho Tyr1158/Tyr1162/Tyr1163)After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Synthetic phosphopeptide derived from the region of IR/IGF1R that contains tyrosines 1158, 1162 and 1163 of the human insulin receptor. The corresponding residues in the IGF1R are 1131, 1135 and 1136. |
| Modification | p-Tyr1158/Tyr1162/Tyr1163 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a dilution of 1/1000. Detects a band of approximately 100 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | INSR phospho Insulin receptor Tyr1158/1162/1163 CD220 Insulin receptor Tyr1158/1162/1163 phospho phospho Y1158/1162/1163 Insulin receptor Phospho Insulin receptor (Tyr1158/1162/1163) Insulin receptor phospho Insulin receptor phospho Y1 |
|---|---|
| Target | Insulin receptor |
| Cellular Localization | Type I membrane protein |
Application
Western Blot using IR/IGF1R [pYpYpY1158/1162/1163] Polyclonal Antibody, Rabbi| Positive Control | CHO-T cells transfected with a vector encoding the human insulin receptor and stimulated with insulin, and 3T3-L1 adipocytes +/- insulin stimulation |
|---|---|
| Application Notes | For WB: Use at a dilution of 1/1000. Detects a band of approximately 100 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3 |
