Mouse monoclonal [83-14] to Insulin Receptor alphaThe human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide linked subunits in a beta-alpha-alpha-beta configuration. The beta subunit (95 kDa) possesses a single transmembrane domain, whereas the alpha subunit (135 kDa) is completely extracellular.
Immunogen
IM-9 lymphocytes followed by purified insulin receptor.
Key Feature
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
83-14
Host Species
Mouse
Tested Applications
ELISAIP
Insulin-like Agonist2 (Order Ab without azide) For ELISA: (Both as coating and reporter) (Order Ab without BSA) For IP: Inhibits ~80% Insulin Binding1 (10nM1,2) Tyrosine Kinase Assay. Optimal dilutions/concentrations should be determined by the researcher.:
Species Reactivity
HumanPigSheepBovine
Concentration
0.2mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
CD220 Insulin receptor alpha HHF5
Target
Insulin receptor alpha
Cellular Localization
Cell membrane
Light Chain Type
kappa
Positive Control
IM-9 lymphocyte cells, Placenta
Application Notes
Insulin-like Agonist2 (Order Ab without azide) For ELISA: (Both as coating and reporter) (Order Ab without BSA) For IP: Inhibits ~80% Insulin Binding1 (10nM1,2) Tyrosine Kinase Assay. Optimal dilutions/concentrations should be determined by the researcher.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.
Storage Buffer
10mM Phosphate buffered saline, pH7.4 containing 0.2% BSA and 0.1% sodium azide. Available BSA and azide free as GTX72314