| Product Name | Insulin Receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [1.B.109] to Insulin ReceptorThe insulin receptor is a tetrameric transmembranous glycoprotein composed of two alpha subunits (120kD) and two beta subunits (90kD). The alpha subunits are disulfide linked, totally extracellular and are the insulin binding site. The beta subunits are transmembranous and contain an intracellular tyrosine kinase domain. The biological effects of insulin are mediated primarily through the insulin receptor. The interaction of insulin with the alpha-subunit of the insulin receptor activates the protein tyrosine kinase of the beta-subunit which then undergoes an autophosphorylation that increases its tyrosine kinase activity. Three adaptor proteins, IRS-1, IRS-2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with SH2 domain-containing signaling proteins. |
| Immunogen | Purified human alpha subunit of insulin receptor. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 1.B.109 |
| Host Species | Mouse |
| Tested Applications | InhibitionIP |
| IP: Use at an assay dependent dilution. Inhib: GTX18177 gives 90% inhibition of insulin binding adipocytes. Shown to activate receptor Kinase in receptor kinase assay. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | CD220 IR Insulin receptor HHF5 |
|---|---|
| Target | Insulin receptor |
| Tissue Specificity | Recognizes alpha subunit of human insulin receptor. |
| Cellular Localization | Type I cell membrane protein. |
| Application Notes | IP: Use at an assay dependent dilution. Inhib: GTX18177 gives 90% inhibition of insulin binding adipocytes. Shown to activate receptor Kinase in receptor kinase assay. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None. Constituents: PBS. pH 7.0 |
Data sheet for OM259610