| Product Name | Insulin Receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [29B4] to Insulin ReceptorThe insulin receptor is a tetrameric transmembranous glycoprotein composed of two alpha subunits (120kD) and two beta subunits (90kD). The alpha subunits are disulfide linked, totally extracellular and are the insulin binding site. The beta subunits are transmembranous and contain an intracellular tyrosine kinase domain.The biological effects of insulin are mediated primarily through the insulin receptor. The interaction of insulin with the alpha-subunit of the insulin receptor activates the protein tyrosine kinase of the beta-subunit which then undergoes an autophosphorylation that increases its tyrosine kinase activity. Three adaptor proteins, IRS-1, IRS-2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with SH2 domain-containing signaling proteins. |
| Immunogen | Partially purified placental insulin receptor |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 29B4 |
| Host Species | Mouse |
| Tested Applications | IPNeut |
| For IP: Use at a concentration of 1-2 μg/sample. For Neut: Use at an assay dependent dilution. Not suitable for WB nor IHC-P. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | CD220 IR Insulin receptor HHF5 |
|---|---|
| Target | Insulin receptor |
| Positive Control | HepG2 cells |
|---|---|
| Application Notes | For IP: Use at a concentration of 1-2 μg/sample. For Neut: Use at an assay dependent dilution. Not suitable for WB nor IHC-P. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 50 mM sodium phosphate buffer, 0.2% gelatin containing =0.1% sodium azide |
Data sheet for OM259611