| Product Name | Insulin receptor beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [CT - 1] to Insulin Receptor betaIR is a receptor tyrosine kinase which mediates the biological activities of insulin by regulating multiple signaling pathways through activation of a series of phosphorylation cascades. The receptor is a disulfide linked heterotetrameric glycoprotein consisting of two alpha subunits and two beta subunits arranged in the following configuration: beta-alpha-alpha-beta. The alpha subunits each contain insulin binding sites and are entirely extracellular in localization. The beta subunits each possess an extracellular domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Binding of insulin to the alpha subunits induces a conformation change in the receptor which activates the kinase domain, stimulating tyrosine autophosphorylation of the receptor and tyrosine phosphorylation of at least five different insulin receptor substrates designated IRS-1-4, and Shc. |
| Immunogen | Synthetic peptide: GKKNGRILTLPRSNPS, corresponding to amino acids 1367-1382 of Human Insulin Receptor. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | CT-1 |
| Host Species | Mouse |
| Tested Applications | EIAIP |
| EIA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Target | Insulin receptor beta |
|---|---|
| Tissue Specificity | This antibody is specific for the approximately 95 kDa IR and shows no cross-reactivity with insulin like growth factor (IGF) receptors. Phosphorylation of the threonine residue within this sequence (corresponding to threonine 1348) decreases the affinity of this antibody for IR by approximately 100 fold. This antibody reacts with autophosphorylated IR, unoccupied IR, and the insulin IR complex. It has no effect on insulin-stimulated autophosphorylation. |
| Cellular Localization | Type I membrane protein. |
| Light Chain Type | kappa |
| Positive Control | IM-9 lymphocytes |
|---|---|
| Application Notes | EIA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Preservative: 0.09% Sodium Azide; Constituents: 0.2% BSA, PBS. pH 7.4 |
