| Product Name | Integrin alpha 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [J143] to Integrin alpha 3The URO panel of antibodies was developed to immuno-anatomically dissect the nephron from glomerulus to bladder urothelium - these antibodies detect various cell surface antigens specific to segments of the renal tubule. Several have been shown to occur as tissue related markers of renal cancers.This antibody reacts predominantly with adherent cell types and is absent from cells growing in suspension, e.g. leukemia, lymphoma, lymphoblastoid, or other hematopoietic cell lines. In tissues, the URO1 antigen is expressed by glomeruli, the basal layer of transitional epithelium and skin, and the basement membrane of glands. All bladder tumors express URO1, as do most kidney tumors and other epithelial tumors (e.g. colon, ovary, lung and breast). Astrocytoma, melanoma, sarcoma, and lymphoma specimens are negative for URO1 expression. |
| Immunogen | 253J bladder cancer cell line. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | J143 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-F |
| ICC: Use at a dilution of 1/40. IHC-Fr: Use at a dilution of 1/40. Pretreatment is not required. Fix for 10 minutes in 4°C acetone. For optimal staining, the primary antibody should be incubated 20-60 minutes at room temperature. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Integrin alpha3 |
|---|---|
| Target | Integrin alpha 3 |
| Tissue Specificity | This antibody recognizes the alpha 3 subunit of VLA3 (ECMRI - extracellular matrix receptor I, URO1), which has been shown to be related to the chicken fibronectin/laminin receptor. VLA proteins are part of the integrin family of cell adhesion molecules, and the distribution of VLA3 in cells and tissues suggests a possible relationship with cellular adhesion.This antibody detects glycoproteins of 140, 120 and 30kDa. |
| Positive Control | Kidney |
|---|---|
| Application Notes | ICC: Use at a dilution of 1/40. IHC-Fr: Use at a dilution of 1/40. Pretreatment is not required. Fix for 10 minutes in 4°C acetone. For optimal staining, the primary antibody should be incubated 20-60 minutes at room temperature. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 1% BSA, 0.01M PBS |
