| Product Name | Intra-Acrosomal Protein (Human) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [Hs-36] to Intra-Acrosomal Protein (Human)One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction. |
| Immunogen | Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | Hs-36 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| ICC/IF: Use at 10 µg/mL. Staining technique: Membrane permeabilization (acetone) is essential: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Intra Acrosomal Protein IntraAcrosomal Protein |
|---|---|
| Target | Intra-Acrosomal Protein (Human) |
| Tissue Specificity | The antibody Hs-36 reacts with human intra-acrosomal proteins (60kDa, 25kDa) associated with the membranes of the acrosomal vesicle. |
| Application Notes | ICC/IF: Use at 10 µg/mL. Staining technique: Membrane permeabilization (acetone) is essential: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. Do not freeze. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
