Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-IRF3 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-IRF3mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded breast tissue using anti-IRF3 mouse monoclonal antibody. ( Dilution 1:50)Application
Anti-IRF3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with IRF3Application
Immunohistochemical staining of paraffin-embedded colon tissue using anti-IRF3mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF3.Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-IRF3mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-IRF3mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-IRF3mouse monoclonal antibody. ( Dilution 1:50)| Product Name | IRF3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2G3] to IRF3Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains. |
| Immunogen | Protein expressed in 293T cell transfected with human IRF3 expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 2G3 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | IRF3 |
|---|---|
| Tissue Specificity | is specific for human IRF3. |
| Cellular Localization | Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm. |
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-IRF3 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-IRF3mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded breast tissue using anti-IRF3 mouse monoclonal antibody. ( Dilution 1:50)
Application
Anti-IRF3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with IRF3
Application
Immunohistochemical staining of paraffin-embedded colon tissue using anti-IRF3mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF3.
Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-IRF3mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-IRF3mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-IRF3mouse monoclonal antibody. ( Dilution 1:50)| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
