Application
Immunocytochemistry of IRGC in A20 cells with IRGC antibody at 2.5 ug/ml.Application
Western blot analysis of IRGC in mouse brain tissue lysate with IRGC antibody at (A) 1 and (B) 2 ug/ml.Application
Immunofluorescence of IRGCb in A20 cells with IRGCb antibody at 20 μg/mL.| Product Name | IRGC antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to IRGCImmunity-related GTPases (IRG) (also known as p47 GTPases) are a family of GTPase proteins found in vertebrates, which play critical roles in mediating innate resistance to intracellular pathogens. IRG genes have been found in a number of mammals and lower species including mice, rats, zebrafish and humans. Most of the mouse genes contain interferon-stimulated response elements which mediate transcriptional activation by IFNs. In humans, only two IRG genes have been found: human IRGC encodes a full-length IRG protein that, like the mouse homologue, is constitutively expressed in testis, while human IRGM encodes a considerably truncated protein that is constitutively expressed in cultured cells including some macrophage cell lines. As the two human genes IRGC and IRGM are not subject to IFN control, it has been suggested that the host resistance mechanism supported by IRG proteins in the mouse is lacking in humans. Two isoforms of IRGC are known to exist; this antibody will recognize both isoforms. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IRGC Immunityrelated GTPase cinema 1 Interferoninducible GTPase5 Interferon-inducible GTPase 5 Interferoninducible GTPase 5 CINEMA Immunity-related GTPase cinema 1 Iigp5 Immunityrelated GTPase cinema1 Interferon inducible GTPase 5 |
|---|---|
| Target | IRGC |
| Tissue Specificity | This antibody is specific for IRGC C-Terminus |
Application
Immunocytochemistry of IRGC in A20 cells with IRGC antibody at 2.5 ug/ml.
Application
Western blot analysis of IRGC in mouse brain tissue lysate with IRGC antibody at (A) 1 and (B) 2 ug/ml.
Application
Immunofluorescence of IRGCb in A20 cells with IRGCb antibody at 20 μg/mL.| Positive Control | Blocking Peptide: |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
