Application
Immunocytochemistry of IRGC in A20 cells with IRGC antibody at 2.5 μg/mL.Application
Immunofluorescence of IRGCb in A20 cells with IRGCb antibody at 20 μg/mL.Application
Western blot analysis of IRGC in mouse brain tissue lysate with IRGC antibody at (A) 1 and (B) 2 μg/mL.| Product Name | IRGC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IRGC Antibody: Immunity-related GTPases (IRG) (also known as p47 GTPases) are a family of GTPase proteins found in vertebrates, which play critical roles in mediating innate resistance to intracellular pathogens. IRG genes have been found in a number of mammals and lower species including mice, rats, zebrafish and humans. Most of the mouse genes contain interferon-stimulated response elements which mediate transcriptional activation by IFNs. In humans, only two IRG genes have been found: human IRGC encodes a full-length IRG protein that, like the mouse homologue, is constitutively expressed in testis, while human IRGM encodes a considerably truncated protein that is constitutively expressed in cultured cells including some macrophage cell lines. As the two human genes IRGC and IRGM are not subject to IFN control, it has been suggested that the host resistance mechanism supported by IRG proteins in the mouse is lacking in humans.1) Taylor GA. IRG proteins: key mediators of interferon-regulated host resistance to intracellular pathogens. Cell Microbiol.2007; 9:1099-107. |
| Immunogen | IRGC antibody was raised against a 12 amino acid synthetic peptide near the carboxy terminus of human IRGC. The immunogen is located within the last 50 amino acids of IRGC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| IRGC antibody can be used for detection of IRGC by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRGC |
|---|---|
| Alternative Names | IRGC Antibody: IFGGE IRGC1 Iigp5 CINEMA R30953_1 IIGP5 Interferon-inducible GTPase 5 Immunity-related GTPase cinema 1 |
| Sequence Similarities | 2) Cat. No. 17-208 - A-20 Cell Slide |
| Tissue Specificity | Two isoforms of IRGC are known to exist; this antibody will recognize both isoforms. |
Application
Immunocytochemistry of IRGC in A20 cells with IRGC antibody at 2.5 μg/mL.
Application
Immunofluorescence of IRGCb in A20 cells with IRGCb antibody at 20 μg/mL.
Application
Western blot analysis of IRGC in mouse brain tissue lysate with IRGC antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
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| Application Notes | IRGC antibody can be used for detection of IRGC by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | IRGC antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IRGC Antibody is supplied in PBS containing 0.02% sodium azide. |
