| Product Name | JAK3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [A1 - 14 - 16] to JAK3JAK 3 (also designated L JAK) is a member of the Janus family of protein tyrosine kinases along with JAK 1, JAK 2, or Tyk 2. Subsequent to activation, these kinases function to activate various members of the Stat family of transcription factors by phosphorylation on critical tyrosine regulatory sites. JAK 3 is activated in response to IL-3 and IL-4 in T cells and myeloid cells. JAK 3 activation requires the serine rich membrane proximal region of the IL 2 receptor beta chain, but does not require the acidic domain that is required for association and activation of Src family kinases. Expression of JAK 3 is restricted to NK and T cells. |
| Immunogen | Recombinant full length protein, corresponding to amino acids 1076-1100 of Mouse JAK3. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | A1-14-16 |
| Host Species | Mouse |
| Tested Applications | IHC-PIPWB |
| Immunoprecipitation (with protein A): use at a concentration of 2μg / mg of lysate. Western Blotting: use at a concentration of 1 - 2μg / ml for 2hrs at RT. Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 2 - 4μg / ml for 30 min at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of ~115 kDa, which corresponds to the predicted molecular weight of JAK 3. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 min.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Purification | Protein A |
| Gene Synonyms | Janus Kinase 3 L-JAK |
|---|---|
| Target | JAK3 |
| Tissue Specificity | This antibody does not cross react with JAK 1, JAK 2 or Tyk 2. |
| Cellular Localization | Cytoplasmic and Nuclear |
| Light Chain Type | kappa |
| Positive Control | Jurkat cells. Tonsil tissue sections. |
|---|---|
| Application Notes | Immunoprecipitation (with protein A): use at a concentration of 2μg / mg of lysate. Western Blotting: use at a concentration of 1 - 2μg / ml for 2hrs at RT. Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 2 - 4μg / ml for 30 min at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of ~115 kDa, which corresponds to the predicted molecular weight of JAK 3. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 min.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
