Application
Immunohistochemistry of JPH3 in human brain tissue with JPH3 antibody at 2.5 ug/ml.Application
Western blot analysis of JPH3 in Daudi cell lysate with JPH3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of JPH3 in Human Brain cells with JPH3 antibody at 20 ug/mL.| Product Name | JPH3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to JPH3Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain. In the CNS, both JPH3 and JPH4 are expressed throughout neural sites and contribute to the subsurface cistern formation in neurons. Mice lacking both JPH3 and JPH4 subtypes exhibit serious symptoms such as impaired learning and memory and are accompanied by abnormal nervous functions. A repeat expansion in JPH3 is associated with Huntington disease-like 2. At least two isoforms of JPH3 are known to exist. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Junctophilin3 JPH3 JP3 Junctophilin 3 HDL2 JP-3 TNRC22 JP 3 |
|---|---|
| Target | JPH3 |
| Tissue Specificity | This antibody is specific for JPH3 C-Terminus |
Application
Immunohistochemistry of JPH3 in human brain tissue with JPH3 antibody at 2.5 ug/ml.
Application
Western blot analysis of JPH3 in Daudi cell lysate with JPH3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of JPH3 in Human Brain cells with JPH3 antibody at 20 ug/mL.| Positive Control | Blocking Peptide: |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
