| Product Name | Kaiso antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2T15] to KaisoTranscriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. May recruit the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. Contributes to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. |
| Immunogen | Recombinant fusion protein (N-terminal), corresponding to amino acids 103 - 584 of mouse Kaiso. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2T15 |
| Host Species | Mouse |
| Tested Applications | GSAIHCIPWB |
| For GSA: Use at an assay dependent dilution. Supershifts a specific methyl-CpG complex. For IHC: Use at a concentration of 1 μg/ml for positive nuclear staining in NIH3T3 cells fixed with 100% methanol. For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 0.5 - 2 μg/ml on RIPA lysates from 3T3/A31 cells (ECL). Detects a band of approximately 97 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDogRatHamster |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | AW260253 E130014G12Rik Kaiso Znf-kaiso Znf kaiso Znfkaiso |
|---|---|
| Target | Kaiso |
| Positive Control | 3T3/A31 cell lysate. Add 2.5µl of 2-mercaptoethanol/100µl of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels. |
|---|---|
| Application Notes | For GSA: Use at an assay dependent dilution. Supershifts a specific methyl-CpG complex. For IHC: Use at a concentration of 1 μg/ml for positive nuclear staining in NIH3T3 cells fixed with 100% methanol. For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 0.5 - 2 μg/ml on RIPA lysates from 3T3/A31 cells (ECL). Detects a band of approximately 97 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 30% Glycerol, 0.15M Sodium Chloride, 0.1M Tris-glycine. pH 7.4 |
