Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using KAP1 antibody at 1:200 dilution.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 7.5% SDS PAGE diluted at 1:10000Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000Application
KAP1 antibody [N1N2], N-term detects KAP1 protein at nucleus on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. KAP1 antibody [N1N2], N-term diluted at 1:500.Application
KAP1 antibody [N1N2], N-term detects KAP1 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. KAP1 antibody [N1N2], N-term diluted at 1:500.| Product Name | KAP1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to KAP1 (tripartite motif-containing 28)The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human KAP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (91%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ29029 antibody KAP1 antibody RNF96 antibody TF1B antibody TIF1B antibody TRIM28 antibody transcription intermediary factor 1-beta antibody KRIP-1 antibody E3 SUMO-protein ligase TRIM28 antibody RING finger protein 96 antibody KRAB-interacting |
|---|---|
| Molecular Weight(MW) | 89 kDa (note) |
| Cellular Localization | Nucleus (By similarity) |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using KAP1 antibody at 1:200 dilution.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 7.5% SDS PAGE diluted at 1:10000
Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000
Application
KAP1 antibody [N1N2], N-term detects KAP1 protein at nucleus on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. KAP1 antibody [N1N2], N-term diluted at 1:500.
Application
KAP1 antibody [N1N2], N-term detects KAP1 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. KAP1 antibody [N1N2], N-term diluted at 1:500.| Positive Control | A431 , H1299 , HeLa , HepG2 , NIH-3T3 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
