Application
KAP1 antibody immunoprecipitates KAP1 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of KAP1 protein by 2 μg of KAP1 antibody 7.5% SDS-PAGE The immunoprecipitated KAP1 protein was detected by KAP1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
ChIP assay followed by QPCR on a known KAP1-binding region within the ZNF180 3’UTR (Mol Cell Biol, 2011, Lyengar et al)Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
KAP1 antibody [N3C2], Internal detects KAP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KAP1 protein stained by KAP1 antibody [N3C2], Internal diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using KAP1 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using KAP1 antibody at 1:500 dilution.| Product Name | KAP1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to KAP1 (tripartite motif containing 28)The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human KAP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (91%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ29029 antibody KAP1 antibody RNF96 antibody TF1B antibody TIF1B antibody TRIM28 antibody transcription intermediary factor 1-beta antibody KRIP-1 antibody E3 SUMO-protein ligase TRIM28 antibody RING finger protein 96 antibody KRAB-interacting |
|---|---|
| Molecular Weight(MW) | 89 kDa (note) |
| Cellular Localization | Nucleus (By similarity) |
Application
KAP1 antibody immunoprecipitates KAP1 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of KAP1 protein by 2 μg of KAP1 antibody 7.5% SDS-PAGE The immunoprecipitated KAP1 protein was detected by KAP1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
ChIP assay followed by QPCR on a known KAP1-binding region within the ZNF180 3’UTR (Mol Cell Biol, 2011, Lyengar et al)
Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
KAP1 antibody [N3C2], Internal detects KAP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KAP1 protein stained by KAP1 antibody [N3C2], Internal diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using KAP1 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using KAP1 antibody at 1:500 dilution.| Positive Control | H1299 , HeLa , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
