Application
IHC analysis of mouse dopaminergic neurons using KCa2.3 antibody (red) and Tyrosine hydroxylase antibody (green).Application
WB analysis of rat brain membranes using KCa2.3 antibody at a dilution of 1:200 in the (1) absence or (2) presence of blocking peptide.| Product Name | KCa2.3 / KCNN3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to KCa2.3 / KCNN3Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. This gene belongs to the KCNN family of potassium channels. It encodes an integral membrane protein that forms a voltage-independent calcium-activated channel, which is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. This gene contains two CAG repeat regions in the coding sequence. It was thought that expansion of one or both of these repeats could lead to an increased susceptibility to schizophrenia or bipolar disorder, but studies indicate that this is probably not the case. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011] |
| Immunogen | Peptide DTSGHFHDSGVGDLDEDPKC, corresponding to amino acid residues 2-21 of human KCa2.3 (SK3) (Accession Q9UGI6). Intracellular, N-terminus. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | hSK3 SK3 potassium intermediate/small conductance calcium-activated channel subfamily N member 3 SKCA3 KCa2.3 KCNN3 |
|---|---|
| Target | KCa2.3 / KCNN3 |
Application
IHC analysis of mouse dopaminergic neurons using KCa2.3 antibody (red) and Tyrosine hydroxylase antibody (green).
Application
WB analysis of rat brain membranes using KCa2.3 antibody at a dilution of 1:200 in the (1) absence or (2) presence of blocking peptide.| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS), pH 7.4, 1% BSA and 0.05% NaN3. |
