| Product Name | KDEL receptor antibody [KR-10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [KR - 10] to KDEL ReceptorRetention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product. |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to amino acids 192-212 of Cow KDEL Receptor. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | KR-10 |
| Host Species | Mouse |
| Tested Applications | ICC/IF,IP,WB |
| For ICC: Use at a dilution of 1:900 - 1:1,100. For IP: Use at a dilution of 1:400 - 1:600. For WB: Use at a dilution of 1:200 - 1:300. Predicted molecular weight: 25 kDa. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human,Mouse,Dog,Monkey,Pig,Rat,Hamster,Rabbit,Xenopus,Bovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC139648 |
|---|---|
| Target | KDEL receptor |
| Cellular Localization | Integral membrane protein. |
| Application Notes | For ICC: Use at a dilution of 1:900 - 1:1,100. For IP: Use at a dilution of 1:400 - 1:600. For WB: Use at a dilution of 1:200 - 1:300. Predicted molecular weight: 25 kDa. Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | None |
