Application
Western blot analysis using KDM4A mAb against HEK293 (1) and KDM4A (AA: 500-705)-hIgGFc transfected HEK293 (2) cell lysateApplication
Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and human larynx cancer tissues (right) using KDM4A mouse mAb with DAB stainingApplication
Immunofluorescence analysis of NTERA-2 cells using KDM4A mouse mAb ( green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin| Product Name | KDM4A antibody [5H1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5H1] to KDM4AThis gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq, Apr 2009] |
| Immunogen | The immunogen corresponding to human KDM4A expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5H1 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHCIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.ELISA:1:10000 WB:1/500 - 1/2000 IHC:1/200 - 1/1000 ICC:1/200 - 1/1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | JHDM3A , TDRD14A , JMJD2 , JMJD2A |
|---|---|
| Target | KDM4A |
| Molecular Weight(MW) | 120 kDa (note) |
Application
Western blot analysis using KDM4A mAb against HEK293 (1) and KDM4A (AA: 500-705)-hIgGFc transfected HEK293 (2) cell lysate
Application
Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and human larynx cancer tissues (right) using KDM4A mouse mAb with DAB staining
Application
Immunofluorescence analysis of NTERA-2 cells using KDM4A mouse mAb ( green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.ELISA:1:10000 WB:1/500 - 1/2000 IHC:1/200 - 1/1000 ICC:1/200 - 1/1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
