Rabbit Polyclonal antibody to KEAP1This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]
Immunogen
KEAP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human KEAP1.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAICC/IFWB
WB: 1 - 2 ug/ml. IF: 20 ug/ml:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
INrf2 KLHL19 kelch-like ECH-associated protein 1
Target
KEAP1
Tissue Specificity
At least two isoforms of KEAP1 are known to exist.
Application
Application
Western blot analysis of KEAP1 in human lung tissue lysate with KEAP1 antibody at (A) 1 and (B) 2 ug/mL.
Application
Immunofluorescence of KEAP1 in human lung tissue with KEAP1 antibody at 20 ug/mL.
Application Notes
WB: 1 - 2 ug/ml. IF: 20 ug/ml:
Additional Information
Form
Liquid
Storage Instructions
Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.