Immunohistochemical
ImmunohistochemicalWestern blot
Host: Rabbit Target Name:KEAP1 Sample Tissue:HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:1.25ug/mL Peptide Concentration: 1.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12%| Product Name | Rabbit anti-KEAP1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | INrf2, KIAA0132, KLHL19, MGC10630, MGC1114, MGC20887, MGC4407, MGC9454 |
| Immunogen | The immunogen for anti-KEAP1 antibody: synthetic peptide directed towards the C terminal of human KEAP1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | KEAP1 |
|---|---|
| Gene Synonyms | INrf2 KIAA0132 KLHL19 MGC10630 MGC1114 MGC20887 MGC4407 MGC9454 |
| Gene Full Name | Kelch-like ECH-associated protein 1 |
| Gene Summary | KEAP1 contains KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase.This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. |
| Alternative Names | INrf2 KIAA0132 KLHL19 MGC10630 MGC1114 MGC20887 MGC4407 MGC9454 |
| Molecular Weight(MW) | 70kDa |
| Sequence | 624 amino acids |
Immunohistochemical
Immunohistochemical
Western blot
Host: Rabbit Target Name:KEAP1 Sample Tissue:HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:1.25ug/mL Peptide Concentration: 1.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12%| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM107035